Detergent Surfactant Raw Material CAS 75621-03-3 3-((3-Cholamidopropyl)dimethylammonium)-1-propanesulfonate CHAPS CAS NO.75621-03-3

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CHAPS is a non-denatured zwitterionic detergent for membrane biochemistry. Can be used to solubilize membrane proteins as well as to break down protein-protein interactions. CHAPS has a low micellar molecular weight (6,150) and a high critical micelle concentration (6-10 mM) and can therefore be removed from the sample by dialysis. It is also suitable for dissolving proteins in isoelectric focusing and two-dimensional electrophoresis. CHAPS is commonly used for non-denaturing (urea-free) isoelectric focusing and has been found to yield excellent resolution for certain subcellular preparations and vegetable proteins. The concentration between 2-4% (w / v) is usually used for isoelectric focusing gels.
It is an important PH stable reagent in biotic experiments.Appropriate PH is obtained by mixing mild acid and its conjugate base.Many biological responses happen in neutral condition (usually PH 6-8),so the buffering range is required to be in PH 6-8. In addition, the formof acid-base of buffer should not chelate with some metallic ions.