China (Mainland)
Reducing Agent Raw Material CAS 51805-45-9 Tris(2-carboxyethyl)phosphine Hydrochloride / TCEP.HCl CAS NO.51805-45-9
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- Min.Order: 1 Gram
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- Available Specifications:
Pharmaceutical Grade(1-10)KilogramPharmaceutical Grade(11-100)Kilogram
- Product Details
Keywords
- TCEP.HCl
- Tris(2-carboxyethyl)phosphine Hydrochloride
- CAS 51805-45-9
Quick Details
- ProName: Reducing Agent Raw Material CAS 51805-...
- CasNo: 51805-45-9
- Molecular Formula: C9H16ClO6P
- Appearance: White powder
- Application: Mainly use in pharmaceutical intermedi...
- DeliveryTime: Abiut 10 days
- PackAge: Aluminum foil bag,fluorinated bottles,...
- Port: Shenzhen port
- ProductionCapacity: 10 Metric Ton/Month
- Purity: 99%
- Storage: Store in cool & dry place. Keep away f...
- Transportation: 1) Door to Door Service by DHL/FEDEX/E...
- LimitNum: 1 Gram
- Moisture Content: <0.1%
- Impurity: <0.1%
Superiority
Details
|
Product name |
TCEP.HCL Tris(2-Carboxyethyl)PhosphineHydrochloride |
|
CAS |
51805-45-9 |
|
Appearance & Physical State |
White Crystalline Solid |
|
Density |
1.041 g/mL at 25ºC |
|
Melting Point |
177ºC |
|
Boiling Point |
519.4ºC at 760 mmHg |
|
Flash Point |
267.9ºC |
|
Refractive Index |
n20/D 1.367 |
|
Storage Condition |
2-8ºC |
Information
TCEP (tris(2-carboxyethyl)phosphine) is a reducing agent frequently used in biochemistry and molecular biology applications. It is often prepared and used as a hydrochloride salt (TCEP-HCl) with a molecular weight of 286.65 gram/mol. It is soluble in water and available as a stabilized solution at neutral pH and immobilized onto an agarose support to facilitate removal of the reducing agent.
Application
TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis.
Compared to the other two most common agents used for this purpose (dithiothreitol and β-mercaptoethanol), TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent (in the sense that TCEP does not regenerate--the end product of TCEP-mediated disulfide cleavage is in fact two free thiols/cysteines), more hydrophilic, and more resistant to oxidation in air. It also does not reduce metals used in immobilized metal affinity chromatography.
TCEP is particularly useful when labeling cysteine residues with maleimides. TCEP can keep the cysteines from forming di-sulfide bonds and unlike dithiothreitol and β-mercaptoethanol, it will not react as readily with the maleimide.However, TCEP has been reported to react with maleimide under certain conditions.
TCEP is also used in the tissue homogenization process for RNA isolation.
FAQ
