USP GMP manufacturer Tris(2-carboxyethyl)phosphine hydrochloride CAS NO.51805-45-9
- FOB Price: USD: 500.00-500.00 /Kilogram Get Latest Price
- Min.Order: 1 Gram
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- Available Specifications:
Top grade(1-1000)Kilogram
- Product Details
Keywords
- phosphine hydrochloride
- 51805-45-9
- Pharmaceutical Intermediates
Quick Details
- ProName: USP GMP manufacturer Tris(2-carboxyeth...
- CasNo: 51805-45-9
- Molecular Formula: C9H16ClO6P
- Appearance: White powder
- Application: Pharmaceutical Intermediates
- DeliveryTime: 12-15 days
- PackAge: Foil Bag, According to Client's Requir...
- Port: SHANGHAI
- ProductionCapacity: 100 Kilogram/Day
- Purity: 99%
- Storage: store in cool and dry place
- Transportation: FedEx DHL EMS or by Sea
- LimitNum: 1 Gram
- Moisture Content: <0.1%
- Impurity: <0.1%
- Grade: Medicine Grade
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Details
USP GMP manufacturer Tris(2-carboxyethyl)phosphine hydrochloride
Product Description
Product name | TCEP.HCL/ Tris(2-Carboxyethyl)PhosphineHydrochloride |
CAS NO. | 51805-45-9 |
Category | Pharmaceutical Raw Materials,Active Pharmaceutical Ingredients,Bulk Drugs,Fine Chemicals. |
Appearance & Physical State: | White Crystalline Solid |
Density: | 1.041 g/mL at 25ºC |
Melting Point: | 177ºC |
Boiling Point: | 519.4ºC at 760 mmHg |
Flash Point: | 267.9ºC |
Refractive Index: | n20/D 1.367 |
Storage Condition: | 2-8ºC |
More information of 51805-45-9
TCEP (tris(2-carboxyethyl)phosphine) is a reducing agent frequently used in biochemistry and molecular biology applications. It is often prepared and used as a hydrochloride salt (TCEP-HCl) with a molecular weight of 286.65 gram/mol. It is soluble in water and available as a stabilized solution at neutral pH and immobilized onto an agarose support to facilitate removal of the reducing agent.
Storage:It should be stored in a well-closed container at low temperature, keep away from moisture, heat and light.
Application of 51805-45-9
TCEP is often used as a reducing agent to break disulfide bonds within and between proteins as a preparatory step for gel electrophoresis.
Compared to the other two most common agents used for this purpose (dithiothreitol and β-mercaptoethanol), TCEP has the advantages of being odorless, a more powerful reducing agent, an irreversible reducing agent (in the sense that TCEP does not regenerate--the end product of TCEP-mediated disulfide cleavage is in fact two free thiols/cysteines), more hydrophilic, and more resistant to oxidation in air. It also does not reduce metals used in immobilized metal affinity chromatography.
TCEP is particularly useful when labeling cysteine residues with maleimides. TCEP can keep the cysteines from forming di-sulfide bonds and unlike dithiothreitol and β-mercaptoethanol, it will not react as readily with the maleimide.However, TCEP has been reported to react with maleimide under certain conditions.
TCEP is also used in the tissue homogenization process for RNA isolation.
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