Tris(Hydroxymethyl)aminomethane Hydrochloride Tris-hcl Buffer CAS NO.1185-53-1 Tris Hcl CAS NO.1185-53-1

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Tris-hcl is commonly used as a buffer component for phenol extractions of DNA or RNA and a buffering component of separating and stacking gels in the characterization of protein products by SDS-PAGE. Tris, Tris-hcl has also been used with urea as a method for antigen retrieval in immunohistochemistry. In smooth muscles Tris, . In the crystalization of porcine pancreatic elastase with use of Tris, Tris-hcl, the Tris, Tris-hcl buffer was observed to induce conformational change and crystal-packing contraction. Tris, Tris-hcl has also been incorporated in coelomic fluid and in Cortland medium to store unfertilized rainbow trout eggs.